Bacteria homologus to Aeromonas capable of microcystin degradation

Water blooms dominated by cyanobacteria are capable of producing hepatotoxins known as microcystins. These toxins are dangerous to people and to the environment. Therefore, for a better understanding of the biological termination of this increasingly common phenomenon, bacteria with the potential to degrade cyanobacteria-derived hepatotoxins and the degradative activity of culturable bacteria were studied. Based on the presence of the mlrA gene, bacteria with a homology to the Sphingopyxis and Stenotrophomonas genera were identified as those presenting potential for microcystins degradation directly in the water samples from the Sulejów Reservoir (SU, Central Poland). However, this biodegrading potential has not been confirmed in in vitro experiments. The degrading activity of the culturable isolates from the water studied was determined in more than 30 bacterial mixes. An analysis of the biodegradation of the microcystin-LR (MC-LR) together with an analysis of the phylogenetic affiliation of bacteria demonstrated for the first time that bacteria homologous to the Aeromonas genus were able to degrade the mentioned hepatotoxin, although the mlrA gene was not amplified. The maximal removal efficiency of MC-LR was 48%. This study demonstrates a new aspect of interactions between the microcystin-containing cyanobacteria and bacteria from the Aeromonas genus.The authors would like to acknowledge the European Cooperation in Science and Technology, COST Action ES 1105 “CYANOCOST - Cyanobacterial blooms and toxins in water resources: Occurrence, impacts and management” for adding value to this study through networking and knowledge sharing with European experts and researchers in the field. The Sulejów Reservoir is a part of the Polish National Long- Term Ecosystem Research Network and the European LTER site

Lasery w koncepcji przemysłu 4.0

In this paper is presented the general idea of the industrial revolution which is called Industry 4.0. The main assumptions and an analysis of the laser use and requirements of lasers for flexible and agile production are presented. Examples of the different technological possibilities when lasers are joined with other machines and IT systems are described.W pracy przedstawiono ogólną koncepcję rewolucji przemysłowej nazywanej „Przemysł 4.0”. Opisano jej podstawowe założenia oraz przeanalizowano zastosowania laserów w kontekście wymagań elastycznej i zwinnej produkcji. Przedstawiono przykład różnorodnych możliwości technologicznych, jakie dają lasery wspomagane innymi obrabiarkami oraz systemami informatycznymi

Bacteria homologus to Aeromonas capable of microcystin degradation

Water blooms dominated by cyanobacteria are capable of producing hepatotoxins known as microcystins. These toxins are dangerous to people and to the environment. Therefore, for a better understanding of the biological termination of this increasingly common phenomenon, bacteria with the potential to degrade cyanobacteria-derived hepatotoxins and the degradative activity of culturable bacteria were studied. Based on the presence of the mlrA gene, bacteria with a homology to the Sphingopyxis and Stenotrophomonas genera were identified as those presenting potential for microcystins degradation directly in the water samples from the Sulejów Reservoir (SU, Central Poland). However, this biodegrading potential has not been confirmed in in vitro experiments. The degrading activity of the culturable isolates from the water studied was determined in more than 30 bacterial mixes. An analysis of the biodegradation of the microcystin-LR (MC-LR) together with an analysis of the phylogenetic affiliation of bacteria demonstrated for the first time that bacteria homologous to the Aeromonas genus were able to degrade the mentioned hepatotoxin, although the mlrA gene was not amplified. The maximal removal efficiency of MC-LR was 48%. This study demonstrates a new aspect of interactions between the microcystin-containing cyanobacteria and bacteria from the Aeromonas genus

Tajik Basin and Southwestern Tian Shan, Northwestern India-Asia Collision Zone: 3. Preorogenic to Synorogenic Retro-foreland Basin Evolution in the Eastern Tajik Depression and Linkage to the Pamir Hinterland

©2020. American Geophysical Union. All Rights Reserved. The Tajik basin archives the orogenic evolution of the Pamir hinterland. Stratigraphic-sedimentologic observations from Cretaceous-Pliocene strata along its eastern margin describe the depositional environment and basin-formation stages in reaction to hinterland exhumation and basin inversion. During the Late Cretaceous-Eocene (preorogenic stage: ~100–34 Ma), a shallow-marine to terrestrial basin extended throughout Central Asia. An alluvial plain with influx of conglomerate bodies (Baljuvon Formation) indicates a first pulse of hinterland erosion and foreland-basin formation in the late Oligocene-early Miocene (synorogenic stage Ia: ~34–23 Ma). Further hinterland exhumation deposited massive alluvial conglomerates (Khingou Formation) in the early-middle Miocene (synorogenic stage Ib: ~23–15 Ma). Westward thickening growth strata suggest transformation of the Tajik basin into the Tajik fold-thrust belt in the middle-late Miocene (synorogenic stage IIa: ~15–5 Ma). Increased water supply led to the formation of fluvial mega-fans (Tavildara Formation). Latest Miocene-Pliocene shortening constructed basin morphology that blocked sediment bypass into the central basin from the east (Karanak Formation), triggering drainage-system reorganization from transverse to longitudinal sediment transport (synorogenic stage IIb: < ~5 Ma). Accelerated shortening (~27–20 Ma) and foreland-directed collapse (~23–12 Ma) of Pamir-plateau crust loaded the foreland and induced synorogenic stages Ia and Ib. Coupling of Indian and Asian cratonic lithospheres and onset of northward and westward delamination/rollback of Asian lithosphere (i.e., lithosphere of the Tajik basin) beneath the Pamir at ~12–11 Ma transformed the Tajik basin into the Tajik fold-thrust belt (synorogenic stage IIa). The timing of the sedimentologically derived basin reconfiguration matches the thermochronologically derived onset of Tajik-basin inversion at ~12 Ma

Tajik Basin and Southwestern Tian Shan, Northwestern India-Asia Collision Zone: 1. Structure, Kinematics, and Salt Tectonics in the Tajik Fold-and-Thrust Belt of the Western Foreland of the Pamir

International audienceSurface, seismic, and borehole data characterize the Neogene-Recent Tajik fold-and-thrust belt of the Tajik basin. The basin experienced little sub-detachment basement deformation, acting as a rigid foreland plate during the Pamir orogeny. The Tajik fold-and-thrust belt contains variable thinskinned structural styles, changing along and across strike as a function of the thickness and facies of Upper Jurassic evaporites, which constitute the basal detachment, and the influence of the surrounding thickskinned belts. The southern Tajik fold-and-thrust belt shows regularly spaced, salt-cored, thrusted detachment anticlines that transition northward into imbricated thrust sheets grouped in oppositely verging stacks facing each other across a common footwall syncline. The width of the fold-and-thrust belt decreases northeastward accommodated by the Ilyak fault, a lateral ramp developed over a seismically active dextral basement fault. The southeastern Tajik fold-and-thrust belt contains massive subaerial salt sheets, formed by squeezing of preexisting salt diapirs. The salt-tectonic domain originates from a local depocenter within the Late Jurassic Amu Darya-Tajik evaporitic basin. Serial cross sections, integrating the structural geometries, yielded minimum thinskinned shortening oriented at~90°to the India-Asia convergence direction, increasing from~93 km in the south to~148 km in the center, and dropping tõ 22 km in the northeast; total shortening-including the foreland buttress-is ≥170 km. Most of the shortening in the central-southern Tajik fold-and-thrust belt occurred by hinterland-vergent, high-displacement back thrusts. The Pamir played a dominant role in the transfer of shortening to the sedimentary infill of the Tajik basin with the Tian Shan acting as a semi-passive buttress

Tajik Basin and Southwestern Tian Shan, Northwestern India-Asia Collision Zone: 3. Preorogenic to Synorogenic Retro-foreland Basin Evolution in the Eastern Tajik Depression and Linkage to the Pamir Hinterland

The Tajik basin archives the orogenic evolution of the Pamir hinterland. Stratigraphic-sedimentologic observations from Cretaceous-Pliocene strata along its eastern margin describe the depositional environment and basin-formation stages in reaction to hinterland exhumation and basin inversion. During the Late Cretaceous-Eocene (preorogenic stage: ~100–34 Ma), a shallow-marine to terrestrial basin extended throughout Central Asia. An alluvial plain with influx of conglomerate bodies (Baljuvon Formation) indicates a first pulse of hinterland erosion and foreland-basin formation in the late Oligocene-early Miocene (synorogenic stage Ia: ~34–23 Ma). Further hinterland exhumation deposited massive alluvial conglomerates (Khingou Formation) in the early-middle Miocene (synorogenic stage Ib: ~23–15 Ma). Westward thickening growth strata suggest transformation of the Tajik basin into the Tajik fold-thrust belt in the middle-late Miocene (synorogenic stage IIa: ~15–5 Ma). Increased water supply led to the formation of fluvial mega-fans (Tavildara Formation). Latest Miocene-Pliocene shortening constructed basin morphology that blocked sediment bypass into the central basin from the east (Karanak Formation), triggering drainage-system reorganization from transverse to longitudinal sediment transport (synorogenic stage IIb: < ~5 Ma). Accelerated shortening (~27–20 Ma) and foreland-directed collapse (~23–12 Ma) of Pamir-plateau crust loaded the foreland and induced synorogenic stages Ia and Ib. Coupling of Indian and Asian cratonic lithospheres and onset of northward and westward delamination/rollback of Asian lithosphere (i.e., lithosphere of the Tajik basin) beneath the Pamir at ~12–11 Ma transformed the Tajik basin into the Tajik fold-thrust belt (synorogenic stage IIa). The timing of the sedimentologically derived basin reconfiguration matches the thermochronologically derived onset of Tajik-basin inversion at ~12 Ma